Vitamin E, especially d-α-tocopherol, is of great importance in animal nutrition and possesses high biological activity. Young green forages and wheat seedlings have a high content of tocopherols whereas grains have a low content of α-tocopherol, resulting in lower vitamin E activity. Root crops and low-fat feeds such as skim milk and whey contain little tocopherols. Consequently, the more stable dl-α-tocopheryl acetate is often used in animal nutrition as a substitute for the easily oxidized d-α-tocopherol. Vitamin E is essential for cellular metabolism, acting as an antioxidant to protect unsaturated fatty acids and vitamin A from oxidation, regulating muscle and carbohydrate metabolism, reproductive health, and hormonal balance. Vitamin E deficiency can lead to problems in livestock such as muscle atrophy, heart disease, and reduced reproductive capacity, significantly affecting health and productive performance.